By J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner
This handbook is a pragmatic advisor, to be used through laboratory employees in health and wellbeing centres and district hospitals, to the techniques to be in acquiring specimens, setting apart and determining micro organism, and assessing their resistance to antibiotics. It covers bacteriological research of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. specific consciousness is given to the necessity for qc of all laboratory approaches. an inventory of media and reagents wanted for the isolation and identity of the most typical bacterial pathogens is incorporated, including a sign in their relative significance for the middleman laboratory. This checklist is meant for model to neighborhood conditions.
This moment variation has been up to date in lots of components, together with a drastically greater part on stool specimens and a brand new part on serological assessments.
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Extra resources for Basic Lab Procedures in Clinical Bacteriology
5). Be sure to identify each rectangular area with the number or name of the patient. Remove a second strip from the container and repeat the procedure exactly, making a second imprint identical to the first. Once the plate is completely inoculated with duplicate impressions, it should be incubated at 35–37 ∞C, and the colonies resulting from each dip-strip imprint counted. With the help of Fig. 5 it is possible to convert the average number of colonies for each pair of dip-strips to the number of bacteria per ml of urine.
Preliminary identification Growth on MacConkey agar is suggestive of Enterobacteriaceae and should be further identified using the methods and media recommended for enteric pathogens. Colonies of Gram-positive cocci with a narrow zone of b-haemolysis may be S. agalactiae (group B streptococci). This should be confirmed with the reverse CAMP test (page 101). Flat colonies with a concave centre and a slight green zone of a-haemolysis are probably S. pneumoniae. For confirmation, a 6-mm optochin disc should be placed on a blood agar plate heavily inoculated with a pure culture of the suspected strain.
Sonnei). S. dysenteriae and S. flexneri are the most commonly isolated Shigella species in developing countries, while S. sonnei is the most commonly isolated species in developed countries. At least six different classes of diarrhoea-producing Escherichia coli have been identified: enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohaemorrhagic or verotoxin-producing E. coli (EHEC or VTEC), enteroinvasive E. coli (EIEC), enteroadhesive E. coli (EAEC), and enteroaggregative E.
Basic Lab Procedures in Clinical Bacteriology by J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner